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雄性Ｗｉｓｔａｒ大鼠２１只，分为输精管结扎组（ＶＧ）和假手术组（ＳＯＧ）。术后４个月，以胸腺细胞增殖法检测腹腔Ｍφ和睾丸组织匀浆上清中ＩＬ－１样物质的活性。结果表明，（１）ＩＬ－１物质活性，随睾丸匀浆上清稀释倍数增加而增高，而不同稀释倍数的腹腔Ｍφ培养上清的各测定值均于同一水平。（２）输精管结扎对腹腔Ｍφ培养上清的ＩＬ－１样物质活性无影响，但明显地提高了睾丸匀浆上清的ＩＬ－１样物质活性。本实验结果提示，睾丸组织中存在有ＩＬ－１样物质的抑制因子。
Twenty-one male Wistar rats were divided into vasectomy group (VG) and sham operation group (SOG). At 4 months after operation, the activity of IL-1 in the peritoneal Mφ and testis homogenate supernatants was detected by thymocyte proliferation assay. The results showed that: (1) The activity of IL-1 increased with the increase of dilution of testis homogenate supernatant, while the measured value of intraperitoneal Mφ culture supernatant at different dilutions were in the same level. (2) Vasectomy had no effect on the IL-1-like activity in the peritoneal Mφ culture supernatant, but significantly increased the IL-1-like activity of testis homogenate supernatant. The results suggest that testicular tissue IL-1-like substances exist in the inhibition factor.