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取中国四川产的大黄(雅黄)710g,50%含水丙酮室温提取,乙酸乙酯-水萃取,水层酸化,放置去深棕色沉淀.取上清提取物经浓缩后进行Sephadex LH-20柱层析,水洗脱,得总苷,甲醇溶解,溶解部分再经ODS-Q3柱层析,用含醋酸(10ml/L)的5～15%乙腈溶液洗脱,得大黄酸苷(Rheinosides)A(14.18g),B(3.64g),C(2.64g)和D(4.56g).经NMR分析和化学降解,鉴定A和B为一对差向异构体,结构为:8-O-β-D-萄萄糖基-10-羟基-10-C-β-D-葡萄糖基大黄-9-蒽酮;C和D为另一对差向异构体,结构为:8-O-β-D-葡萄糖基-10-C-β-D-葡萄糖基大黄9-蒽酮,它们的绝对构型正在鉴定中.
Take Chinese rhubarb (Astragalus) 710g produced in Sichuan, extracted with 50% aqueous acetone at room temperature, extracted with ethyl acetate-water, acidified with water and placed on a dark brown precipitate.The supernatant extract was concentrated and subjected to Sephadex LH-20 column Chromatography, water elution, the total glycosides, methanol dissolved, the dissolved part by ODS-Q3 column chromatography, with acetic acid (10ml / L) of 5 ~ 15% acetonitrile elution to give Rheinosides (Rheinosides) A (14.18g), B (3.64g), C (2.64g), and D (4.56g). NMR and chemical degradation identified A and B as a pair of epimers with the structure: 8-O -β-D-glucosyl-10-hydroxy-10-C-β-D-glucosyl rhubarb-9-anthrone; C and D are the other epimer of the 8-O -β-D-glucosyl-10-C-β-D-glucosyl rhubarb 9-anthrone, their absolute configuration is under identification.