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探讨糖尿病大鼠血管组织糖基化终产物 (AGEs)含量与其受体 (RAGE)和细胞间粘附因子 1(ICAM 1)表达的关系。复制糖尿病大鼠模型 ,采用荧光法、RT PCR及原位杂交方法检测主动脉及心肌组织的AGEs含量以及RAGE和ICAM 1基因的表达。发现糖尿病大鼠主动脉和心肌组织AGEs含量升高 (P <0 0 1) ;RAGE和ICAM 1基因表达增强 (P <0 0 5～ 0 0 1) ;AGEs含量与RAGE及ICAM 1呈明显正相关 (P <0 0 1) ;氨基胍治疗可缓解上述指标的变化。提示AGEs可诱导RAGE和ICAM 1的表达。推测AGEs RAGE相互作用是引起糖尿病血管内皮细胞功能紊乱和损伤的关键环节。
To investigate the relationship between the levels of advanced glycation end products (AGEs) and the expressions of RAGE and ICAM 1 in diabetic rats. The diabetic rat model was duplicated. The contents of AGEs and the expression of RAGE and ICAM-1 in the aorta and myocardium were detected by fluorescence, RT-PCR and in situ hybridization. The results showed that AGEs in the aorta and myocardium of diabetic rats were increased (P <0.01), the expression of RAGE and ICAM 1 was increased (P <0.05-0.01), the contents of AGEs were positively correlated with RAGE and ICAM 1 (P <0.01); aminoguanidine treatment can alleviate the above indicators of change. Tip AGEs can induce RAGE and ICAM 1 expression. It is speculated that the AGEs RAGE interaction is a key factor that causes dysfunction and damage of diabetic vascular endothelial cells.