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本文用组织化学方法研究乙酰苯肼(APH)所造成的动物体内急性溶血性贫血对大鼠脾脏的影响,系统地观察了脾脏内酶的活性变化,并用显微分光光度计对巨噬细胞内抗氟化物酯酶的活性进行了定量测定。实验结果表明: 1.注射APH后,随着脾小体缩小和淋巴细胞的减少,脾脏白髓和红髓的琥珀酸脱氢酶、谷氨酸脱氢酶活性逐渐降低,第9天最低,第13天有所恢复。 2.实验动物脾红髓的葡萄糖-6-磷酸酶活性于第3～6天时有所下降,第9天上升至接近正常,第13天稍高于正常水平。 3.注射APH后,脾红髓的酸性磷酸酶和三磷酸腺苷酶活性于实验第6天开始明显增强;非特异性酯酶和抗氟化物酯酶的活性于第3天开始增强,并与注射APH的次数相平行。显微分光光度计测定结果表明,实验动物脾脏巨噬细胞内的抗氟化物酯酶活性于第3,6,9,13天时分别为正常动物的7.4、9.5、20.3和9.4倍。上述酶活性的增强是与巨噬细胞吞噬消化功能的增强相适应的。
In this paper, histochemistry was used to study the effect of acute hemolytic anemia caused by acetophenhydrazine (APH) on the spleen of rats. The changes of enzyme activities in the spleen were systematically observed. Fluoride esterase activity was quantified. The experimental results showed that: 1.After APH injection, the succinate dehydrogenase and glutamate dehydrogenase activity of spleen white pulp and red pulp decreased gradually with the spleen constriction and lymphocyte decrease, the lowest was on the 9th day, The 13th day has been restored. The activity of glucose-6-phosphatase of spleen red pulp of experimental animals decreased from the 3rd to 6th day, rose to nearly normal on the 9th day, slightly higher than the normal level on the 13th day. The activity of acid phosphatase and adenosine triphosphatase in splenic red pulp began to increase significantly on the 6th day after APH injection. The activity of non-specific esterase and fluoride esterase increased on the 3rd day, The number of parallel. The results of the spectrophotometer showed that the activities of fluoride-resistant esterase in the spleen macrophages of experimental animals were 7.4, 9.5, 20.3 and 9.4 times higher than normal animals on the 3rd, 6th, 9th and 13th days, respectively. The above enzyme activity is enhanced with phagocytosis of macrophages to adapt to the enhancement.