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[Objective] To detect the aradecoside A contents in Aralia plants in different production areas by HPLC. [Method] Chromatographic column was Kromasil C18 column (250 mm × 4.6 mm, 5 μm). Mobile phase was acetonitrile-0.05% phosphoric acid solution (V/V, 35∶65); detection wavelength was 205 nm; column temperature was 30 ℃; flow rate was 0.8 ml/min; injection volume was 20 μl. [Result] Aradecoside A had good linear relationship with peak area between 0.632 5-25.30 μg/ml (r=0.999 9). The average recovery rate was 97.97% and RSD was 1.02%. [Conclusion] This method was accurate, sensitive, and repeatable and could be used for the quality control of root barks of Aralia plants.
[Objective] To detect the aradecoside A contents in Aralia plants in different production areas by HPLC. [Method] Chromatographic column was Kromasil C18 column (250 mm × 4.6 mm, 5 μm). Mobile phase was acetonitrile-0.05% phosphoric acid solution V / V, 35:65); detection wavelength was 205 nm; column temperature was 30 ° C .; flow rate was 0.8 ml / min; injection volume was 20 μl. [Result] Aradecoside A had good linear relationship with peak area between 0.632 5 -25.30 μg / ml (r = 0.999 9). The average recovery rate was 97.97% and RSD was 1.02%. [Conclusion] This method was accurate, sensitive, and repeatable and could be used for the quality control of root barks of Aralia plants.