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【目的】初步分析鼠衣原体(Chlamydia muridarum,Cm)致不同遗传背景小鼠生殖道病变的原因,探讨炎症细胞动态变化在衣原体感染致不同人群病变差异的可能机制。【方法】用2×105 Inclusion forming units(IFUs)/小鼠的Cm剂量经子宫角(Intrauterine inoculation,iu)途径分别感染DBA/2J鼠和A/J鼠;在感染60 d处死两组小鼠,肉眼观察其输卵管水肿程度,显微镜下观察评估输卵管的扩张程度及炎症反应程度;每组小鼠在感染后3、7、14、21、28、35、42、49、56、60 d分别取阴道分泌物并检测其中衣原体包涵体的数量;在第14天每组各处死5只小鼠,检测上生殖道(输卵管及卵巢)组织匀浆中衣原体包涵体数量及炎症因子水平;同时,在3、28、35 d每组分别处死5只小鼠,显微镜下鉴定病变组织炎症细胞种类及变化。【结果】两组小鼠在感染60 d肉眼观察下的生殖道病变率有明显统计学差异,且两组小鼠病变组织的输卵管扩张程度也具有统计学差异,但炎症反应程度评分无统计学差异;DBA/2J与A/J两组小鼠在阴道分泌物的衣原体包涵体检测及小鼠阳性检出率中并无显著性差异;感染后14 d两组小鼠在上生殖道组织匀浆中的包涵体检出量无统计学差异,但某些炎症因子水平具有统计学差异;感染早期两组小鼠病变组织中嗜中性粒细胞浸润增加,但无明显差异,第28天DBA/2J小鼠病变组织中出现较多嗜酸性粒细胞,并在第35天与A/J小鼠形成统计学差异。【结论】DBA/2J与A/J小鼠在以iu方式感染等量Cm后,引起小鼠的生殖道病变差异可能与嗜酸性粒细胞参与的炎症反应有关。
【Objective】 The aim of this study is to analyze the causes of genital tract lesions caused by Chlamydia muridarum (Cm) in mice of different genetic backgrounds and to explore the possible mechanism of the dynamic changes of inflammatory cells in the pathological changes of different populations caused by Chlamydial infection. 【Methods】 DBA / 2J mice and A / J mice were infected with 2 × 105 Inclusion forming units (IFUs) / Mice by intrauterine inoculation (iu) respectively. Two groups of mice were sacrificed 60 days after infection , The extent of tubal edema was observed with the naked eye, the degree of tubal expansion and the degree of inflammatory reaction were observed and evaluated under the microscope. At 3,7,14,21,28,35,42,49,56,60 d after infection, Vaginal secretions were collected and the number of inclusion bodies of chlamydia was detected. On the 14th day, 5 mice were killed in each group to detect the number of chlamydial inclusion bodies and inflammatory cytokines in the upper genital (tubal and ovarian) tissue homogenates. At the same time, 3, 28, 35 d, 5 mice were sacrificed in each group. The types and changes of inflammatory cells in the lesion were identified under the microscope. 【Results】 The incidence of genital tract lesions was significantly different between the two groups at 60 days after infection, and the degree of tubal dilation in the two groups was also statistically different, but there was no statistical difference in the degree of inflammatory reaction Differences between the two groups of mice in DBA / 2J and A / J detection of vaginal secretions of chlamydial inclusion bodies detected and the positive rate of the mice did not show significant differences; 14 days after infection, the two groups of mice in the genital tract homogenization There was no significant difference in the amount of inclusion bodies in the plasma between the two groups, but the levels of some inflammatory cytokines were statistically different. In the early stage of infection, neutrophil infiltration was increased in the lesion tissues of the two groups, but there was no significant difference. On the 28th day, DBA / 2J mice showed more eosinophils in the diseased tissues and formed a statistically significant difference with the A / J mice on the 35th day. 【Conclusion】 Differences in genital tract lesions induced by eosinomycin in DBA / 2J and A / J mice may be related to the inflammatory response of eosinophils.