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目的探讨Notch信号通路在血小板衍生生长因子(PDGF)-BB诱导人胰腺癌细胞(HPAC)增殖过程中的作用。方法 MTT法检测PDGF-BB及γ-secretase抑制剂4’,6-二脒基-2-苯基吲哚(DAPT)对HPAC增殖活力的影响;Western blotting检测细胞Notch蛋白表达水平的变化;染色质免疫共沉淀(CHIP)检测Notch下游靶基因转录水平的变化。结果 PDGF-BB显著促进HPAC增殖,提高细胞Notch表达水平,增强Notch靶基因的转录;不同剂量的DAPT及血小板衍生生长因子受体(PDGFR)抑制剂处理细胞,显著反转了PDGF-BB的作用。结论 Notch信号通路在PDGF-BB诱导胰腺癌细胞增殖中起到重要作用。
Objective To investigate the role of Notch signaling pathway in the proliferation of human pancreatic cancer cells (HPAC) induced by platelet-derived growth factor (PDGF) -BB. Methods The effects of PDGF-BB and γ-secretase inhibitor 4 ’, 6-diamidino-2-phenylindole (DAPT) on the proliferation of HPAC were detected by MTT assay. The changes of Notch protein expression were detected by Western blotting. Immunoprecipitation (CHIP) was used to detect the transcriptional level of Notch downstream target genes. Results PDGF-BB significantly promoted the proliferation of HPAC, increased the expression of Notch, and enhanced the transcription of Notch target genes. DAPT and PDGFR inhibitors at different doses significantly reversed the effect of PDGF-BB . Conclusion Notch signaling pathway plays an important role in PDGF-BB-induced proliferation of pancreatic cancer cells.