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为了检测肉鸡组织中黄曲霉毒素B1(AFB1)的残留量,试验采用柱前衍生-高效液相色谱(HPLC)的分析方法,并用该方法对样品进行检测。结果表明:AFB1在0.5~50.0μg/L范围内具有良好的线性关系,相关系数大于0.999,检出限为0.1μg/kg,信噪比(S/N)=3∶1。在0.5μg/kg、1.0μg/kg、2.0μg/kg 3个添加水平下肝脏组织平均回收率为91.0%~107.0%,肌肉组织平均回收率为94.0%~105.4%,相对标准偏差(RSD)分别为0.8%~1.7%、1.2%~2.0%。说明本方法具有灵敏性、准确性和可重复性,可对动物组织样中AFB1残留进行定性和定量检测。
In order to detect aflatoxin B1 (AFB1) residues in broiler tissues, a pre-column derivatization-high performance liquid chromatography (HPLC) analysis method was used to test the samples. The results showed that AFB1 had a good linearity in the range of 0.5-50.0 μg / L, the correlation coefficient was greater than 0.999, the detection limit was 0.1 μg / kg and the S / N ratio was 3:1. The average recoveries of liver tissue were 91.0% ~ 107.0% and the average recoveries of muscle tissue were 94.0% ~ 105.4% with the three addition levels of 0.5μg / kg, 1.0μg / kg and 2.0μg / kg. The relative standard deviations Respectively 0.8% ~ 1.7%, 1.2% ~ 2.0%. This method shows the sensitivity, accuracy and repeatability of qualitative and quantitative detection of AFB1 residues in animal tissue samples.