β—actin基因启动子驱动的草鱼生长激素基因cDNA—“全鱼”基因 pCAgcGHc用显微注射方法导入四倍体鱼卵 ,获得了生长快 ,个体硕壮的转基因四倍体鱼。 2 4 0日龄时 ,转基因鱼平均体重为 30 2 7g ,是对照鱼的 3 1倍 ,平均体长是对照的 1 34倍 ,并可从部分转基因雄鱼挤出精液 ,而对照鱼尚无此现象。对 19尾转基因四倍体雄鱼的精液和尾鳍DNA做PCR检测 ,外源基因的阳性率分别为 94 7%和 52 6%。最后 ,展望了转基因四倍体鱼在转基因鱼产业化方面的应用前景 β-actin gene promoter-driven grass carp growth hormone gene cDNA- “whole fish” gene pCAgcGHc by microinjection method into the tetraploid fish eggs, get fast growth, individual robust transgenic tetraploid fish. At 240 days of age, the average body weight of transgenic fish was 30 27g, which was 31 times that of the control fish and average body length was 34 times that of the control, and sperm were extruded from some transgenic males while the control fish still had no This phenomenon. PCR was performed on the sperm and caudal fin DNA of 19 transgenic tetraploids. The positive rates of foreign genes were 94.7% and 52.6%, respectively. Finally, the application prospects of transgenic tetraploid fish in the industrialization of transgenic fish are prospected