论文部分内容阅读
调查了安徽茶区茶树丛枝菌根真菌(AMF)资源分布情况,为菌根生物技术在茶产业中的应用提供了具有应用价值的菌种资源.采用醋酸-墨水染色法观察茶树丛枝菌根(AM)的侵染率、侵染级数、侵染强度和菌根类型;采用湿筛法获得AMF孢子,进行形态学鉴定.结果表明:在安徽茶区,AMF能够侵入茶树根系形成典型的共生体,侵染率在36%~95%,侵染级数均在3级以上,侵染强度大;从安徽茶树根际土中共鉴定出8属36种AMF,其中缩管柄囊霉是优势种,网状球囊霉、刺无梗囊霉、孔窝无梗囊霉、詹氏无梗囊霉、双网无梗囊霉和凹坑无梗囊霉是常见种,褐色管柄囊霉、疣突管柄囊霉、毛氏无梗囊霉、近明管柄囊霉、瑞氏无梗囊霉、空洞无梗囊霉、晕环球囊霉、细齿无梗囊霉、地管柄囊霉、幼套球囊霉、蜜色无梗囊霉、稀有内养囊霉是稀有种,其余17种为少见种;不同采样地茶树根际AMF群落相似性系数(0.14~0.55)较低,多属于低和中等水平;相关性分析表明,孢子密度与侵染率呈显著正相关,种的丰度与侵染率和总球囊霉素相关土壤蛋白呈显著正相关.安徽茶区茶树根系存在典型的AM结构,其根际AMF多样性丰富,为开发茶树专用AMF肥料提供了丰富的菌种资源.
This paper investigated the distribution of arbuscular mycorrhizal fungi (AMF) resources in tea plantations in tea area of Anhui Province, and provided valuable fungal resources for the application of mycorrhizal biotechnology in the tea industry.The arbuscular mycorrhizal fungi (AM), infection stages, infection intensity and mycorrhizal type, AMF spores were obtained by wet sieving method, and morphological identification was carried out.The results showed that AMF could invade the root system of tea plant in the typical tea plantation Of the symbionts, the infection rate of 36% to 95%, the infection levels are above the third grade, the intensity of infection; from Anhui rhizosphere soil identified 8 genera and 36 kinds of AMF, Is the dominant species, Mesophyll glomerulus, abscess immature mold, hole juvenile Saccharomyces efflorescence, jenniferous Campylobacter sargassum, Stenolomycetes and pits without rime terrestrial cyst is common species, brown tube handle Groove mold, Warts stem tube gynoecium, Commelina abscess mildew, tube close to the next tube mold, Wisteria stem jujube mold, empty termitomyrum mold, Halocystis glomerulus, juvenile Serratia delphilis, to Tuberosid cyst, young sets of balloon mold, honey-colored juvenile Saccharomyces gosvatica, Rare Inner Saccharomyces cerevisiae is a rare species, and the remaining 17 kinds of rare species; different sampling sites of tea rhizosphere AMF community The similarity coefficient (0.14 ~ 0.55) was lower and mostly belonged to the lower and middle level. Correlation analysis showed that there was a significant positive correlation between the spore density and the infection rate. The abundance, Protein had a significant positive correlation.The tea plantation in the tea area of Anhui Province had a typical AM structure with rich AMF diversity in its rhizosphere and provided abundant germ resources for the development of tea plant-specific AMF fertilizers.