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目的：了解结肠癌缺失基因（ＤＣＣ基因）在原发肝癌发生发展中的作用。方法：采用多聚酶链延伸反应－限制性片段长度多态性分析（ＰＣＲ－ＲＦＬＰ）方法对３０例外科手术切除原发肝癌标本ＤＣＣ基因杂合缺失（ＬＯＨ）进行分析。结果：２８例信息个体中检出ＬＯＨ７例，占２５．０％。ＤＣＣ基因ＬＯＨ与临床病理参数和ＨＢＶ感染无显著相关。结论：ＤＣＣ基因ＬＯＨ可能参与了原发肝癌的发生发展。
Objective: To understand the role of DCC gene in the development of primary liver cancer. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to analyze the heterozygosity loss (LOH) of DCC gene in 30 primary surgically resected specimens. RESULTS: 7 cases of LOH were detected in 28 individuals, accounting for 25.0%. There was no significant correlation between DCC gene LOH and clinicopathological parameters and HBV infection. Conclusion: The LOH gene of DCC may participate in the occurrence and development of primary liver cancer.