论文部分内容阅读
目的:探讨肿瘤转移抑制基因Kiss-1在舌鳞癌原发灶和淋巴结转移灶的表达。方法:采用免疫组织化学SP法检测59例舌鳞癌标本原发灶癌组织和其中21例发生淋巴结转移的转移灶中Kiss-1蛋白的表达,并分析其与临床病理参数的关系。结果:59例原发灶标本中Kiss-1蛋白的表达率:无淋巴结转移组为97.37%(37/38),有淋巴结转移组为90.47%(19/21),两组相比差异无统计学意义(p>0.05);但有淋巴结转移组的表达强度低于无淋巴结转移组,两组差异有统计学意义(p<0.05)。在21例发生淋巴结转移的病例中,淋巴结转移灶表达率为28.57%(6/21),明显低于原发灶的90.47%(19/21),两组差异有统计学意义(p<0.01)。Kiss-1蛋白表达与患者的年龄、性别、临床分期、组织学分级、嗜烟酒等因素无关(p>0.05)。结论:舌鳞癌中Kiss-1蛋白表达减弱或丢失可能是导致舌鳞癌转移能力增强的因素之一。
Objective: To investigate the expression of Kiss-1, a tumor metastasis suppressor gene, in the primary and lymph node metastases of tongue squamous cell carcinoma. Methods: Immunohistochemical SP method was used to detect the expression of Kiss-1 protein in 59 specimens of primary squamous cell carcinoma of the tongue with squamous cell carcinoma of the tongue and 21 cases of lymph node metastasis. The relationship between the expression of Kiss-1 protein and clinicopathological parameters was analyzed. Results: The expression of Kiss-1 in 59 cases of primary tumor was 97.37% (37/38) in non-lymph node metastasis group and 90.47% (19/21) in lymph node metastasis group, there was no statistical difference between the two groups (P> 0.05). However, the intensity of expression in lymph node metastasis group was lower than that in non-lymph node metastasis group, the difference was statistically significant (p <0.05). In 21 cases of lymph node metastasis, the expression rate of lymph node metastasis was 28.57% (6/21), which was significantly lower than that of primary tumor (90.47%, 19/21), the difference was statistically significant (p <0.01 ). The expression of Kiss-1 was not related to the age, sex, clinical stage, histological grade, alcohol addicted to alcohol and other factors (p> 0.05). Conclusion: The decrease or loss of Kiss-1 protein in tongue squamous cell carcinoma may be one of the factors leading to the enhanced metastasis of tongue squamous cell carcinoma.