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背景:慢性肝病肝纤维化是可逆转的。有研究表明,美洲大蠊提取物具有一定的抗纤维化作用,其对实验性肝纤维化大鼠起保护作用。目的:观察美洲大蠊提取物黏糖氨酸治疗大鼠肝纤维化效果,初步探讨黏糖氨酸抗肝纤维化的作用机制。方法:采用猪血清诱导免疫性肝纤维化模型大鼠,同时采用0.5,0.25,0.10 g/kg的黏糖氨酸灌胃治疗,放射免疫法检测肝纤4项;免疫组织化学检测肝组织转化生长因子β1、组织金属蛋白酶抑制物蛋白表达强度和阳性细胞率;检测不同浓度的黏糖氨酸与转化生长因子β1、组织金属蛋白酶抑制物相关性。结果与结论:(1)美洲大蠊提取物黏糖氨酸能降低层粘连蛋白、Ⅲ型前胶原、Ⅳ型胶原和透明质酸酶水平(P<0.01),降低转化生长因子β1和组织金属蛋白酶抑制物1在肝组织的表达(P<0.01);(2)黏糖氨酸浓度与转化生长因子β1和组织金属蛋白酶抑制物1的蛋白表达呈明显的负相关(|r|>0.9);(3)结果证实,美洲大蠊提取物黏糖氨酸可逆转肝纤维化的改变,其作用机制与黏糖氨酸抑制转化生长因子β1和组织金属蛋白酶抑制物1的表达有关。
Background: Chronic liver disease is reversible in liver fibrosis. Some studies have shown that Periplaneta americana extract has some anti-fibrosis, which play a protective role in experimental liver fibrosis rats. OBJECTIVE: To observe the effect of periococcal extract on rat hepatic fibrosis and to investigate the mechanism of anti-fibrosis induced by viscous glutamate. Methods: The rat model of autoimmune liver fibrosis induced by porcine serum was used. At the same time, 0.5, 0.25 and 0.10 g / kg of mucosin were intragastrically administered, and 4 of them were detected by radioimmunoassay. The liver tissues were detected by immunohistochemistry The expression of growth factor β1, tissue inhibitor of metalloproteinase (MMP) and the percentage of positive cells were measured. The correlations between different concentrations of myosin and transforming growth factor β1 and tissue inhibitor of metalloproteinase were detected. RESULTS AND CONCLUSION: (1) The American cockroach extract myxoine reduced the levels of laminin, type Ⅲ procollagen, type Ⅳ collagen and hyaluronidase (P <0.01), decreased the expression of transforming growth factor β1 and tissue metal (2) There was a significant negative correlation between the concentration of viscosycine and the protein expression of transforming growth factor β1 and tissue inhibitor of metalloproteinase 1 (| r |> 0.9) ; (3) The results confirmed that the Periplaneta americana extract viscosycine can reverse the changes of hepatic fibrosis, the mechanism of which is related to the inhibition of transforming growth factor-β1 and tissue inhibitor of metalloproteinase-1 by viscous myosin.