论文部分内容阅读
目的:采用高效液相色谱法测定余甘子中没食子酸的含量,优化色谱条件,比较3个来源余甘子中没食子酸的含量差异。方法:分别对来自云南大理(2个批号)和福建漳州的余甘子中没食子酸进行含量测定,色谱条件为十八烷基硅烷键合硅胶色谱柱(粒径25μm,柱直径4.6 mm,柱长250 mm);流动相乙腈-0.2%磷酸水溶液(5∶95);等度洗脱;流速1.000 m L/min;检测波长2273nm;柱温30℃,标准曲线外标法。结果:没食子酸在13.010~130.104μg/m L范围内与峰面积有明显线性关系(R=0.999 4);精密度良好[相对标准偏差(RSD)=0.08%];稳定性良好(RSD=0.29%);重现性一般(RSD=4.23%)。对3批来自不同产地批号的余甘子药材进行了没食子酸含量评价,结果显示3个批次(福建20150304、云南20150205、云南20150304)的余甘子中没食子酸含量分别为2.60%、3.38%和2.82%,3个批次的余甘子中没食子酸含量均高于《中国药典》国家标准1.2%。结论:本方法测定余甘子中的没食子酸含量专属性强、精密度好、结果准确可靠。
OBJECTIVE: To determine the content of gallic acid in Phyllanthus emblica by high performance liquid chromatography (HPLC) and optimize the chromatographic conditions. The content of gallic acid in three sources of amla were compared. Methods: The content of gallic acid in Phyllanthus emblica L. from Yunnan Dali (two batches) and Zhangzhou of Fujian Province were determined respectively. The chromatographic conditions were octadecylsilane bonded silica column (diameter 25 μm, column diameter 4.6 mm, column length 250 mm); mobile phase acetonitrile -0.2% phosphoric acid (5:95); isocratic elution; flow rate 1.000 m L / min; detection wavelength 2273 nm; column temperature 30 ° C. Results: There was a significant linear relationship between gallic acid and peak area in the range of 13.010-130.104μg / m L (R = 0.999 4); good precision (relative standard deviation (RSD) = 0.08%]; good stability %); Reproducibility in general (RSD = 4.23%). The results showed that the content of gallic acid in amla in three batches (Fujian 20150304, Yunnan 20150205, Yunnan 20150304) were 2.60%, 3.38% and 2.82 %, Three batches of Phyllanthus emblica content higher than the “Chinese Pharmacopoeia” national standard of 1.2%. Conclusion: The method for the determination of gallic acid in the content of gallic acid specificity, good precision, the results are accurate and reliable.