中药柴胡解热镇痛镇静的有效成分主要是皂甙类。有关柴胡皂甙的含量测定方法,国外报导较多的有比色法、气相层析法、高效液相层析法以及双波专薄层扫描法等,国内报导较少。有采用紫外分光光度法进行测定。笔者采用了双波长薄层扫描法对柴胡属中的北柴胡、狭叶柴胡、柞柴胡、大叶柴胡等四种柴胡中的皂甙进行了含量测定,并作了结果分析,为开发和利用柴胡入药提出了科学依据。 Antipyretic analgesic and sedative effects of Radix Bupleuri Herba are mainly saponins. Regarding the determination method of saikosaponin content, there are many foreign reports such as colorimetric method, gas chromatography, high-performance liquid chromatography, and double-wave special thin-layer scanning method, and there are few domestic reports. Ultraviolet spectrophotometry was used for the determination. The authors used a dual-wavelength thin-layer scanning method to determine the contents of saponins in Radix Bupleuri, Radix Bupleurum, Radix Bupleuri, Radix Bupleurum and other four Radix Bupleuri, and analyzed the results. , for the development and use of Bupleurum medicine for the scientific basis.