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[Objective] To separate and prepare gentiopicroside and sweroside from the extracts of Swertia franchetiana H. Smith by high speed counter-current chromatography (HSCCC). [Method] Chloroform∶ methanol∶ water=4∶4∶2 (V/V/V) as solvent system, the upper one was stationary phase while the lower one was mobile phase; ultrasonic degassed for 20 min; the rotating velocity of 850 r/min; mobile phase flow rate of 2 ml/min; the detective wavelength of 254 nm; the purity of product was identified by polarimeter. [Result] 14 mg gentiopricroside and 27 mg swersoside were obtained from 100 mg crude extracts of Swertia franchetiana H. Smith, the specific rotation [a]tD of which were -196.3° and -126.6°respectively, which suggested the products were of high purity. [Conclusion] The study would lay a feasible way for the large scale preparation of gentiopicroside and sweroside by HSCCC.
[Objective] To separate and prepare gentiopicroside and sweroside from the extracts of Swertia franchetiana H. Smith by high speed counter-current chromatography (HSCCC). [Method] Chloroform: methanol: water = 4: 4: 2 (V / V / V ) as solvent system, the upper one was stationary phase while the lower one was mobile phase; ultrasonic degassed for 20 min; the rotating velocity of 850 r / min; mobile phase flow rate of 2 ml / min; The purity of product was identified by polarimeter. [Result] 14 mg gentiopricroside and 27 mg swersoside were obtained from 100 mg crude extracts of Swertia franchetiana H. Smith, the specific rotation [a] tD of which were-196.3 ° and -126.6 ° respectively, which suggested the products were of high purity. [Conclusion] The study would lay a feasible way for the large scale preparation of gentiopicroside and sweroside by HSCCC.