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目的探讨快速动眼(rapid eye movement,REM)睡眠剥夺后与大鼠认知相关的行为学变化及脑内海马组织中自噬相关蛋白的表达水平。方法健康成年雄性大鼠经过筛选后分为空白对照组(CC组)、环境对照组(TC组)、睡眠剥夺组(SD组),每组各6只;采用改良多平台睡眠剥夺法(modified multiple platform method,MMPM)建立睡眠剥夺模型,连续剥夺5 d后利用Morris水迷宫检测大鼠认知功能;用蛋白质印记法(Western Blot,WB)检测自噬相关微管蛋白(LC3)及SQSTM1/P62的表达水平变化。结果与CC组和TC组比较,SD组大鼠毛色无光泽、易激惹、体重下降(P<0.05)。SD组与其他2组比较,逃逸潜伏期延长、目标象限时间减少(P<0.05)。WB显示SD组与其他2组比较,大鼠脑内海马组织自噬相关蛋白LC3-II表达水平上升,P62水平下降(P<0.05)。CC组与TC组大鼠比较,体重、学习记忆能力、海马组织自噬蛋白表达水平均无明显差异(P>0.05)。结论睡眠剥夺后可损害大鼠学习及记忆功能,海马组织中自噬水平上调提示自噬活动可能参与睡眠剥夺介导的认知功能障碍过程。
Objective To investigate the cognition-related behavioral changes in rats after rapid eye movement (REM) sleep deprivation and the expression of autophagy-related proteins in the hippocampus. Methods Healthy adult male rats were screened and divided into blank control group (CC group), environmental control group (TC group) and sleep deprivation group (SD group), each group had 6 rats in each group. Modified multi-platform sleep deprivation multiple placental matrix metalloproteinase (MMP) -mediated MMP2 (MMP2) was used to establish the sleep deprivation model. After 5 days of continuous deprivation, Morris water maze was used to detect the cognitive function of rats. LC3 and SQSTM1 / P62 expression level changes. Results Compared with CC group and TC group, SD rats had dull coat, irritability and weight loss (P <0.05). Compared with the other two groups, the escape latency of SD group was prolonged and the target quadrant time was decreased (P <0.05). WB showed that compared with the other two groups, the level of LC3-II and the level of P62 in hippocampus of rats in SD group were decreased (P <0.05). There was no significant difference in body weight, learning and memory ability, hippocampus autophagy protein expression between CC group and TC group (P> 0.05). Conclusion Sleep deprivation can impair learning and memory in rats. Upregulation of autophagy in hippocampus suggests that autophagy may be involved in sleep deprivation-mediated cognitive dysfunction.