斑点免疫法,于1982年 Hawkes 等人建立,是仿照分子生物学中点杂交的方法而发展起来的,操作简便、灵敏度高、试验结果可以长期保存,不需特殊仪器设备、成本低等优点。我们应用斑点免疫法对美国进口马铃薯脱毒组培苗“布尔斑克”、“林海”、“夏波蒂”三个品种各1000株,每个品种各取96份样品,采用植物检疫实验所及中国农科院生物技术中 Dot-immunization, established in 1982 by Hawkes et al., Was modeled on the method of molecular hybridization by midpoint hybridization. It is simple, sensitive and can save long-term test results without special equipment and low cost. We applied the dot immunization method to 1000 strains of three varieties of “Burdock”, “Linhai” and “Xia Podi”, which were imported from the United States, and 96 samples were taken from each variety. The plant quarantine experiment And Chinese Academy of Agricultural Sciences in biotechnology