肌苷注射液(供肌肉注射用)卫生部药品标准采用苯甲酸钠为助溶剂,因苯甲酸钠有较强的紫外吸收并与肌苷的紫外吸收相重叠,故采用纸层析分离后进行含量测定,但此法取样量甚微,操作较为繁琐,影响因素多且操作误差较大,本文采用一阶导数分光光度法可消除苯甲酸钠的干扰,不经分离直接测定肌苷的含量,操作简便,结果满意。 Inosine injection (for intramuscular injection) Ministry of Health drug standards using sodium benzoate as a cosolvent, as sodium benzoate has a strong UV absorption and inosine UV absorption overlap, it was determined by paper chromatography after the determination , But this method is very small sample amount, the operation is more cumbersome, many factors and the operation error is greater, in this paper, the first derivative spectrophotometry can eliminate sodium benzoate interference, direct determination of inosine content without separation, easy to operate, Satisfactory results.