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目的 :观察棘球蚴囊内容物攻击兔所至肺功能和形态的损害。建立囊型包虫肺栓塞的动物模型。方法 :取囊型包虫内容物 ,分离出囊砂 ,与囊液配成 5 %的悬浊液。 2 1只家兔 ,分 3组 :Ⅰ :生理盐水组 ,Ⅱ :澄清囊液组 ,Ⅲ :囊砂悬浊液组。每只家兔都置入股动脉导管及股静脉导管。依照上述分组按 2mL/kg体重分别自静脉导管注射生理盐水、澄清囊液或含囊砂的悬浊液。于注射后 ( 5、30、6 0min)动态监测MAP、血气指标及血清血管活性物质(ET - 1、TXB2 、6 -keto -PGF1α)水平的变化。指标测定后 ,行肺部ECT检查。最后取出动物肺脏行光镜病检。结果 :Ⅲ组和Ⅱ组在注射后均出现MAP、血气指标的明显降低 (P <0 .0 5 )和血清TXB2 、6 -keto -PGF1α的升高 (P <0 .0 5 ) ,尤以Ⅲ组最明显 (P <0 .0 5 )。各组动物血清ET - 1水平在注射前后无明显变化 (P >0 .0 1)。ECT显示Ⅲ组动物肺部放射性缺损 ,Ⅱ组动物肺部放射性减弱。Ⅲ组肺脏病检见头节广泛栓塞于肺小动脉、微小动脉中 ,肺脏呈“急性呼吸窘迫综合症样”改变。Ⅱ组肺组织出现淤血、水肿及炎细胞浸润。结论 :Ⅲ组动物基本能够模拟出囊型包虫病肺栓塞的临床表现。囊液中有形成分在肺损害中起着重要的作用。TXB2 、6 -keto -PGF1α参与了肺损伤病理过程。
Objective: To observe the echinococcosis cyst content of rabbit attack to the lung function and morphology damage. Animal models of cystic echinococcosis were established. Methods: Take the contents of hydatid cyst, separate the cyst and mix it with 5% suspension. 21 rabbits were divided into 3 groups: Ⅰ: saline group, Ⅱ: clear cyst fluid group, Ⅲ: cyst suspension group. Each rabbit was placed in femoral and femoral catheters. According to the above group by 2mL / kg body weight, respectively, from the intravenous catheter injection of saline, clarified cyst fluid or cyst fluid suspension. The levels of MAP, blood gas and serum vasoactive substances (ET - 1, TXB2, 6 - keto - PGF1α) were dynamically monitored at 5, 30 and 60 min after injection. After the index determination, lung ECT examination. Finally, remove the animal lung line light microscopy. Results: MAP, blood gas index (P <0.05), and serum TXB2 and 6-keto-PGF1α levels were significantly increased in group Ⅲ and group Ⅱ after injection (P <0.05), especially The third group was the most obvious (P <0.05). The levels of serum ET - 1 in each group had no significant changes before and after injection (P> 0. ECT showed the radioactive defect of the lungs in group Ⅲ, and radioactivity in the lungs of group Ⅱ was weakened. Ⅲ group of lung disease detected head section embolization in pulmonary arterioles, small arteries, the lung was “acute respiratory distress syndrome-like” changes. Ⅱ group of lung tissue congestion, edema and inflammatory cell infiltration. Conclusion: The animals in group Ⅲ can basically simulate the clinical manifestations of pulmonary embolism. Tangible components in cystic fluid play an important role in lung injury. TXB2, 6-keto-PGF1α is involved in the pathological process of lung injury.