Aim:To study the effects of{2-[(3-carboxy-1-oxoprogy 1)amino]-2-deoxy-D-glu-cose(COPADG)on cultured human hepatocellular carcinoma cells(HepG2).Methods:HepG2 cells were cultured in RPMI-1640 medium.Cell proliferation wasdetermined by MTT assay.Apoptosis was determined by fluorescence microscopy,transmission electron microscopy,agarose gel electrophoresis of DNA fragmenta-tion,and flow cytometry.Results:At the concentration ranging between 1-30μmol/L,COPADG potently inhibited the growth and induced apoptosis of HepG2cells.Conclusion:COPADG could effectively induce apoptosis in human hepato-cellular carcinoma cells.More investigations are warranted for the potential useof this compound as a new agent for the non-surgical management of humanhepatocellular carcinoma. Aim: To study the effects of {2 - [(3-carboxy-1-oxoprogy 1) amino] -2-deoxy-D-glucose (COPADG) on cultured human hepatocellular carcinoma cells (HepG2). Methods: HepG2 cells were cultured by RPMI-1640 medium. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry. Results: At the concentration ranging between 1-30 μmol / L, COPADG potently inhibited the growth and induced apoptosis of HepG2 cells. Confc: COPADG could achieve induce apoptosis in human hepato-cellular carcinoma cells. More investigations are warranted for the potential use of this compound as a new agent for the non-surgical management of human hepatocellular carcinoma.