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为在短时期内培养大量的雪旺氏细胞,本文对目前常用的两种培养方法进行了比较。方法:采用植块反复种植法和酶消化法分别进行雪旺氏细胞的体外培养。结果表明两种方法都能得到雪旺氏细胞。但酶消化法所培养的雪旺氏细胞生长一致,相同条件下达到一定细胞数量所需时间短于植块反复种植法。酶消化法比植块反复种植法更适用于大量雪旺氏细胞的培养。
In order to cultivate a large number of Schwann cells in a short period of time, this article compares the two commonly used culture methods. METHODS: Schwann cells were cultured in vitro by repeated plantation and enzyme digestion. The results show that both methods can get Schwann cells. However, the Schwann cells cultured by enzymatic digestion grew in the same manner, and the time required to reach a certain number of cells under the same conditions was shorter than that of repeated plantations. Enzymatic digestion is more suitable than repeated plantation of plants for cultivation of large numbers of Schwann cells.