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采用薄层色谱法对槐定碱和拉马宁碱的离解常数进行了测定,所得结果与文献值基本一致.该方法的特点是:若待测物质是难分离的混合物,可通过选择合适pH的固定相和展开剂,测其离解常数
The dissociation constants of sophoridine and ramanine base were determined by thin layer chromatography. The obtained results are in good agreement with the published data. The method is characterized in that: if the substance to be tested is a mixture difficult to separate, the dissociation constant can be measured by selecting a suitable pH stationary phase and developing solvent