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1目的 探讨慢性乙型肝炎病人外周血单个核细胞 (PBMC)乙型肝炎病毒 (HBV) DNA和血清 HBVDNA的感染情况及其临床意义。 2方法 采用聚合酶链反应 (PCR)技术对 12 8例慢性乙型肝炎病人和 2 0例健康人进行了 PBMC及血清中 HBV DNA检测。 3结果 慢性乙型肝炎病人 PBMC中 HBV DNA的感染率为 6 4.2 % ,和血清 HBV DNA的感染率具有一致性倾向 (χ2 =0 .6 3,P>0 .0 5 ) ,慢性肝病病人 PBMC内 HBV DNA阳性率随肝病病情加重而升高。 4结论 PBMC中 HBV DNA整合在 HBV病毒传播和病情进程中起一定作用 ,检测 PBMC中 HBV DNA可作为判断病情和指导治疗的有用指标。
1 Objective To investigate the infection of hepatitis B virus (HBV) DNA and serum HBVDNA in peripheral blood mononuclear cells (PBMC) from patients with chronic hepatitis B and its clinical significance. Methods Polymerase chain reaction (PCR) was used to detect HBV DNA in PBMC and serum in 12 8 chronic hepatitis B patients and 20 healthy controls. 3 Results The infection rate of HBV DNA in PBMC of patients with chronic hepatitis B was 62.2%, which was consistent with the infection rate of serum HBV DNA (χ2 = 0.63, P> 0.05). The PBMC of patients with chronic liver disease The positive rate of HBV DNA increased with the severity of liver disease. 4 Conclusion PBMC integration of HBV DNA plays a role in the transmission of HBV and progression of the disease. Detection of HBV DNA in PBMC can be used as a useful indicator to judge the condition and guide the treatment.