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目的探讨全反式维甲酸(ATRA)对前列腺癌细胞生长增殖的抑制作用及其与连接蛋白(Cx)43之间的关系。方法不同浓度的ATRA处理体外培养的雄激素非依赖性人前列腺癌细胞株PC3后,分别应用光镜、甲基偶氮唑蓝(MTT)法和集落形成试验分析细胞生长和增殖的抑制情况;蛋白质免疫印迹法(Westernblot)检测PC3细胞ATRA作用前后Cx43的表达。结果ATRA浓度为(10-6~10-4)mol/L时均可有效抑制PC3细胞的增殖(P<0·05),光镜下可见细胞凋亡的特征性改变,抑制呈时间-剂量依赖性。ATRA处理前后PC3的Cx43表达量从无到阳性光带出现,且显色带随ATRA浓度增高而增强。结论ATRA能够抑制前列腺癌细胞的生长和增殖,其机制可能通过上调Cx43的表达来实现的。
Objective To investigate the inhibitory effect of all-trans retinoic acid (ATRA) on the growth and proliferation of prostate cancer cells and its relationship with connexin (Cx) 43. Methods ATRA-treated androgen-independent human prostate cancer cell line PC3 was treated with different concentrations of ATRA. The inhibition of cell growth and proliferation was analyzed by light microscope, MTT assay and colony formation assay, respectively. Western blot was used to detect the expression of Cx43 in PC3 cells before and after ATRA treatment. Results ATRA concentration of (10-6 ~ 10-4) mol / L could effectively inhibit the proliferation of PC3 cells (P <0.05). The apoptotic characteristics of ATRA cells were observed under light microscope. Dependency. The expression of Cx43 in PC3 before and after ATRA treatment appeared from no to positive bands, and the color bands increased with the increase of ATRA concentration. Conclusion ATRA can inhibit the growth and proliferation of prostate cancer cells, the mechanism may be achieved by up-regulating the expression of Cx43.