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为研究温度、光照和营养盐对小新月菱形藻生长的影响,设计了5个温度,3个光照度,各种营养盐各4个浓度以及6个更新率,进行了小新月菱形藻的培养试验。结果表明:小新月菱形藻的最佳生长温度为15～20℃,最佳光照度为5000 lx,氮、磷、硅、铁的最佳浓度依次为300、15、120、1.575 mg/L;当温度高于30℃或者光照度大于10000 lx时,小新月菱形藻均不能生长。对小新月菱形藻进行半连续培养条件的研究发现,小新月菱形藻在6个不同的更新率(25%、30%、35%、40%、45%和50%)条件下均能完成半连续培养。根据不同更新率下的细胞密度和实际生产中的培养密度,建议采收率在40%～45%。
In order to study the effects of temperature, light and nutrients on the growth of Artemisia mincioides, five temperature, three illumination, four concentrations of various nutrients and six renewal rates were designed. Training test. The results showed that the optimal growth temperature was 15-20 ℃ and the optimal illuminance was 5000 lx. The optimal concentrations of nitrogen, phosphorus, silicon and iron were 300, 15, 120 and 1.575 mg / L, respectively. When the temperature is higher than 30 ℃ or light intensity is greater than 10000 lx, the Neocirpus can not grow. Studies on the condition of semi-continuous culture of Artemisia miniteli revealed that Artemisia microphylla can be regenerated at 6 different update rates (25%, 30%, 35%, 40%, 45% and 50%) Complete semi-continuous culture. According to the cell density at different renewal rates and the culture density in actual production, it is suggested that the recovery rate should be between 40% and 45%.