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本研究首次报道了电镜免疫胶体金对水稻内生细菌的定位，用硫酸铵沉淀结合梯度离心提取表面消毒后分离的大田水稻体内优势菌巨大芽孢杆菌的特异性胞内蛋白，制备兔抗血清为金标一抗，进行微波固定的组织超薄切片免疫胶体金的染色电镜观察。组织切片中菌体有大量金颗粒沉积，证明表面消毒后分离的巨大芽孢杆菌为水稻内生细菌，大多寄生在植物组织的胞间隙，偶尔也在胞质内存在
In this study, the localization of endophytic bacteria by electron microscopy immunogold gold was reported for the first time. The specific intracellular protein of Bacillus megaterium, which is the dominant bacterium in field rice, was isolated by ammonium sulfate precipitation combined with gradient centrifugation. The antiserum was Gold standard primary antibody, microwave fixed tissue ultrathin section immunohistochemistry gold staining electron microscopy. Tissue sections in the bacterial deposition of a large number of gold particles to prove that the surface after sterilization of Bacillus megaterium isolated rice endophytic bacteria, mostly parasitic in the interstitial plant tissue, and occasionally also exist in the cytoplasm