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植物实验课中有丝分裂的观察、孚尔根染色技术等多个实验,一般都采用洋葱根尖或蚕豆根尖为实验材料。根尖的培养有水培法、沙培法、土培法和锯末埋培法。这些方法,在根尖的培养过程中因污染和供氧不足,往往引起洋葱和蚕豆的腐烂,根尖变色老化,影响实验效果。近几年来,我们采用塑料泡沫培养法,收到较好效果,现简介如下: 在木箱或花盆等培养容器的底部铺上一层约5厘米厚的土壤,以利保持湿度。把包装用的废弃泡沫磨成直径为0.5~2毫米的碎块,放在培养容器的土壤上面,
Plant experiments in the observation of mitosis, Fügen staining techniques and other experiments, the general use of onion root tips or Vicia faba as the experimental material. Apical training hydroponics, sand culture method, soil culture and sawdust buried cultivation method. These methods, in the root tip of the cultivation process due to pollution and lack of oxygen, often cause the onion and broad bean decay, discoloration aging, affecting the experimental results. In recent years, we have adopted the plastic foam culture method and received good results. The brief introduction is as follows: Lay a layer of soil about 5 cm thick on the bottom of a culture container such as a wooden box or a flower pot to keep the humidity. The packaging of waste foam ground into a diameter of 0.5 to 2 mm pieces, placed on top of the soil in the culture container,