目的研究MUC-1基因表达与肺癌患者术后复发的关系,探讨RT-PCR法评判临床病情演变及预后的可能性。方法运用RTPCR方法,检测与肿瘤发生和转移密切相关的MUC1基因在46例非小细胞肺癌(NSCLC)患者手术切除的病理标本中的异常表达,结合临床及病理分型和5年随访结果进行实验研究。结果MUC1基因在肺癌组织和纵隔组织过量表达与肺癌患者术后复发及5年生存率密切相关。结论RTPCR法用于检测NSCLC患者淋巴结微转移具有临床可行性,检测结果对于肺癌的准确分型、分期,合理选择治疗方案,提高疗效,减少复发和改善预后有重要指导意义。 Objective To investigate the relationship between the expression of MUC-1 gene and the recurrence of lung cancer patients and to explore the possibility of clinical progression and prognosis by RT-PCR. Methods The RTPCR method was used to detect the abnormal expression of MUC1 gene in 46 cases of non-small cell lung cancer (NSCLC) patients, which were closely related to tumorigenesis and metastasis. Combined with clinical and pathological classification and 5-year follow-up results the study. Results The overexpression of MUC1 gene in lung cancer tissues and mediastinal tissues was closely related to the recurrence rate and 5-year survival rate of lung cancer patients. Conclusion The RTPCR method is clinically feasible for the detection of micrometastases in patients with NSCLC. The detection results have important guiding significance for the accurate classification of lung cancer, staging, rational choice of treatment, improvement of curative effect, reduction of recurrence and improvement of prognosis.