目的 :探讨 p16蛋白表达及Ki- 6 7指数 (Ki- 6 7LI)与垂体腺瘤增殖与侵袭生物学特性的关系。 方法 :应用免疫组化染色技术检测 5 7例垂体腺瘤中 p16及Ki 6 7蛋白的表达。 结果 :垂体腺瘤 p16蛋白阳性率为31 6 % ,侵袭性组 p16蛋白阳性率显著低于非侵袭性组 (P <0 0 1)。Ki 6 7LI在侵袭性组和非侵袭性组分别为3 5 3± 1 2 2和 1 86± 0 6 3,两组有显著性差异 (P <0 0 1)。 18例 p16蛋白阳性垂体腺瘤平均Ki 6 7LI显著低于39例 p16蛋白阴性组 (P <0 0 1)。p16蛋白表达率及Ki 6 7LI均与垂体腺瘤激素分泌功能无明显相关性。 结论 :检测 p16蛋白表达及Ki 6 7LI对临床评价垂体腺瘤侵袭性有积极意义 Objective: To investigate the relationship between p16 protein expression and Ki-67 index (Ki-67LI) and biological characteristics of pituitary adenoma proliferation and invasion. Methods: The expression of p16 and Ki 67 protein in 57 pituitary adenomas were detected by immunohistochemical staining. Results: The positive rate of p16 protein in pituitary adenomas was 316%. The positive rate of p16 protein in invasive adenomas was significantly lower than that in non-invasive ones (P <0 01). The Ki 6 7LI in the invasive group and the non-invasive group was 353 ± 112 and 186 ± 036, respectively, and there was a significant difference between the two groups (P < 0 01). The average Ki 67LI of p16 protein-positive pituitary adenomas was significantly lower in 18 cases than in the negative p16 protein group (P < 0.01). The expression rate of p16 protein and Ki 67LI were not significantly correlated with the hormone secretion function of pituitary adenoma. Conclusion : Detection of p16 protein expression and Ki 67 LI are positive for clinical evaluation of pituitary adenoma invasiveness