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目的探讨老年吸烟者肺癌Ｈｒａｓ基因变化的意义。方法对３３例吸烟肺癌和２２例不吸烟肺癌患者采用纤维支气管镜毛刷刷取支气管脱落细胞，应用ＰＣＲＲＦＬＰ方法进行Ｈｒａｓ基因突变检测。结果肺癌中Ｈｒａｓ基因总突变率为５２７％（２９／５５），其中吸烟组肺癌Ｈｒａｓ基因突变率为６６７％，不吸烟组肺癌为３１８％，二者差异有显著性（Ｐ＜００５），吸烟组肺鳞癌患者Ｈｒａｓ基因突变率为７００％，明显高于不吸烟组肺鳞癌患者的１６７％（Ｐ＜００５）。结论提示Ｈｒａｓ基因可能是被烟草激活的一个特殊位点，吸烟是导致Ｈｒａｓ基因突变的重要因素。
Objective To explore the significance of H-ras gene mutation in lung cancer in elderly smokers. Methods Thirty-three patients with lung cancer with lung cancer and 22 patients with non-smoking lung cancer were treated with fiberoptic bronchoscope brush to remove bronchial exfoliated cells. The PCRRFLP method was used to detect Hras gene mutation. Results The total mutation rate of H ras gene in lung cancer was 52.7% (29/55). Among them, the H ras gene mutation rate was 66.7% in the smoking group and 31.8% in the non-smoking group. Significantly (P <0 05), H ras gene mutation rate was 70.0% in patients with lung squamous cell carcinoma in the smoking group, which was significantly higher than 16 7% of lung squamous cell carcinoma patients in the non-smoking group (P <0. ). The results suggest that H ras gene may be a special site activated by tobacco. Smoking is an important factor leading to H ras gene mutation.