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目的:观察银杏叶提取物对慢性马兜铃酸肾病(CAAN)大鼠肾组织纤维化的影响。方法:大鼠30只予关木通水煎剂灌胃12周,制备大鼠马兜铃酸肾病模型,12周后随机分成未治疗组和治疗组。治疗组(n=15)大鼠予银杏叶〔银杏天宝片,300mg/(kg.d)〕混悬液灌胃8周,未治疗组(n=15)予饮用水灌胃8周。另设健康对照组(n=10)。实验20周时处死所有大鼠,留取血、尿、肾组织标本,行血红蛋白、肾功能、尿蛋白、病理、免疫组化检查及逆转录聚合酶链反应(RT-PCR法)测定纤维化相关因子。结果:与未治疗组比较,治疗组血尿素氮(BUN)、肌酐(Scr)明显下降(P<0.05);肾间质纤维化程度减轻;免疫组化结果:治疗组肾组织转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)、纤维连接蛋白(FN)光密度值较未治疗组明显下降(P<0.05);逆转录聚合酶链反应(RT-PCR法)测定肾组织TGF-β1、CTGF的mRNA表达,结果治疗组均明显减低(P<0.05)。结论:银杏叶提取物能够减轻大鼠CAAN肾组织纤维化。
Objective: To observe the effect of Ginkgo biloba extract on renal fibrosis in rats with chronic aristolochic acid nephropathy (CAAN). METHODS: Thirty rats were given Guan Mu Tong decoction orally for 12 weeks to prepare aristolochic acid nephropathy model. After 12 weeks, they were randomly divided into untreated group and treatment group. Rats in the treatment group (n=15) were given intragastric administration of ginkgo biloba (Ginkgo tianbao tablet, 300 mg/(kg.d)) suspension for 8 weeks. Untreated group (n=15) was given drinking water for 8 weeks. Separate healthy control group (n=10). All rats were sacrificed at the 20th week of the experiment. Samples of blood, urine, and kidney were taken. Hemoglobin, renal function, urine protein, pathology, immunohistochemical examination, and reverse transcriptase polymerase chain reaction (RT-PCR) were used to determine fibrosis. Related factors. RESULTS: Compared with the untreated group, blood urea nitrogen (BUN) and creatinine (Scr) were significantly decreased (P<0.05); renal interstitial fibrosis was reduced; immunohistochemistry results: renal tissue transforming growth factor beta 1 in the treated group The optical density of (TGF-β1), connective tissue growth factor (CTGF), and fibronectin (FN) was significantly lower than that of the untreated group (P<0.05); RT-PCR was used to determine the renal tissue. The mRNA expression of TGF-β1 and CTGF was significantly decreased in the treatment group (P<0.05). Conclusion: Ginkgo biloba extract can reduce the renal tissue fibrosis in CAAN rats.