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目的 :探讨冰冻是否引起精子DNA损伤。 方法 :采用改进后的单细胞凝胶电泳法 (SCGE)对 - 80℃条件下保存不同时间的精子DNA进行分析。 结果 :以DNA头部百分比为检测参数 ,方差分析显示 ,对于时间因素 ,P >0 .0 5 ,无统计学差异。 结论 :冰冻不引起精子DNA损伤
Objective: To investigate whether freezing causes DNA damage in sperm. Methods: The sperm DNAs stored at-80 ℃ for different time were analyzed by single cell gel electrophoresis (SCGE). Results: The percentage of DNA was used as the detection parameter. ANOVA showed that there was no significant difference for the time factor (P> 0.05). Conclusion: Frozen does not cause DNA damage in sperm