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目的比较健康女性阴道不同部位pH值、菌群多样性、密集度、优势菌及局部机体反应状态的差异,阴道分泌物取材对临床诊断的指导意义。方法在普查人群中,通过阴道分泌物涂片镜检排除阴道感染的健康妇女30例,分别在阴道侧壁上1/3处、阴道后穹窿、阴道下1/3处三处取材后进行微生态分析。结果不同部位取材后检验差异不大,但是在取材时存在取材量的不同,拭子饱和度后穹窿较多,阴道下1/3次之,尤以阴道上1/3处拭子饱和度最小。菌群密集度和多样性分析三者之间有差异,乳酸杆菌多在上1/3处居多,阴道后穹窿和阴道下1/3处杂菌相对较多。结论诊断性取样应以阴道上1/3侧壁为准,能真正反映阴道菌群的状态及致病菌。同时在分泌物涂片时应以薄均匀为准,以免影响染色的效果。
Objective To compare the difference of pH value, flora diversity, concentration, dominant bacteria and the reaction status of local body in different parts of healthy vagina, and the guidance of vaginal secretions in clinical diagnosis. Methods Thirty healthy women who had vaginal discharge were microscopically screened by vaginal discharge smear. One third of vaginal wall, the posterior fornix and the third vagina were taken from the vulva. Ecological analysis. The results of different parts of the test after the difference is not significant, but there are drawn in the amount of material present when the swab saturation vault after more than 1/3 times the vagina, especially in the vagina on the 1/3 minimum swab saturation . There was a difference between the three groups in the concentration and diversity of the flora. Lactobacillus was mostly in the upper 1/3, while the posterior vaginal fornix and vaginal 1/3 more bacteria were relatively more. Conclusion Diagnostic sampling should be based on the vaginal wall on the 1/3 prevail, can truly reflect the status of vaginal flora and pathogens. At the same time should be secreted smear thin homogeneous subject, so as not to affect the dyeing effect.