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目的:观察海豹油对酒精性肝病的预防作用。方法:采用酒精灌胃制备酒精性肝病大鼠模型。Wistar雄性大鼠60只,随机分为6组,分别为肝损伤模型对照组、空白对照组、阳性对照组及海豹油的低、中、高三个剂量组。各剂量组每天经口灌胃给予海豹油,空白对照组给予蒸馏水,模型对照组给予色拉油,阳性对照组给予海王牌金樽片。于实验的第30天各组大鼠一次性经口给予50%乙醇12 ml/kg(空白对照组动物给蒸馏水),禁食16 h处死动物,采血,剖腹取肝,制备肝匀浆。测定血清中的天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(AIT)、甘油三脂(TG)、胆固醇(TC)和肝组织中的丙二醛(MDA)、谷光甘肽(GSH)、超氧化物歧化酶(SOD)和TG含量并进行病理组织学检查。结果:与模型对照组比较,海豹油3.0 ml/kg剂量组能明显降低血清ALT和肝组织MDA含量,海豹油3.0 ml/kg、6.0 ml/kg剂量组能明显降低酒精性肝病大鼠血清AST、TG、TC含量和肝组织中TG含量,增强SOD酶的活性,并能改善肝细胞脂肪变性。结论:海豹油对大鼠酒精性肝病有预防作用。
Objective: To observe the seal oil on the prevention of alcoholic liver disease. Methods: A rat model of alcoholic liver disease was prepared by alcohol gavage. Sixty male Wistar rats were randomly divided into 6 groups: control group, blank control group, positive control group and low, medium and high doses of seal oil. In each dose group, the oral administration of seal oil was given orally, the blank control group was given distilled water, and the model control group was given the salad oil. The positive control group was given the sea king brand golden bottle tablets. On the 30th day of experiment, rats in each group were orally administered with 50% ethanol 12 ml / kg (blank control group, distilled water), animals were sacrificed 16 h after fasting, blood was collected, and liver was harvested by laparotomy to prepare liver homogenates. Serum aspartate aminotransferase (AST), alanine aminotransferase (AIT), triglyceride (TG), cholesterol (TC) and malondialdehyde (MDA) Peptide (GSH), superoxide dismutase (SOD) and TG content and histopathological examination. Results: Compared with the model control group, SEA 3.0 ml / kg dose group could significantly reduce serum ALT and liver MDA content. Seal oil 3.0 ml / kg, 6.0 ml / kg dose group could significantly reduce the serum AST , TG, TC content and liver tissue TG content, enhance SOD activity, and can improve fatty degeneration of liver cells. Conclusion: Seal oil can prevent alcoholic liver disease in rats.