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目的:研究鹿蹄草多糖LTC-Ⅱ的结构性质。方法:热水提取,乙醇沉淀制备鹿蹄草粗多糖,粗多糖经DEAE-纤维素和Sephacryl S-300 HR柱色谱分离纯化。采用高效凝胶渗透色谱法鉴定纯度并测定相对分子质量;紫外、红外、旋光、完全酸水解、高碘酸氧化及Smith降解、部分酸水解、甲基化分析对纯多糖组分进行结构性质研究。结果:得到一多糖纯组分LTC-Ⅱ,其相对分子质量为22 000 Da。单糖组成为阿拉伯糖、甘露糖、葡萄糖、半乳糖,其摩尔比为35.2∶1.0∶13.4∶4.2,LTC-Ⅱ主链由甘露糖、葡萄糖和半乳糖组成,以(1→6)连接的葡萄糖为主,分支侧链由阿拉伯糖、葡萄糖和半乳糖组成,以(1→5)连接的阿拉伯糖为主,葡萄糖和半乳糖位于分子的末端。结论:首次对鹿蹄草多糖LTC-Ⅱ的化学结构进行较深入的研究。
Objective: To study the structural properties of wintergall polysaccharide LTC-Ⅱ. Methods: Hot water extraction and ethanol precipitation were used to prepare crude polysaccharide of Hippophae rhamnoides. The crude polysaccharide was separated and purified by DEAE-cellulose and Sephacryl S-300 HR column chromatography. The purity of pure polysaccharides was determined by high performance gel permeation chromatography (GPC) and the relative molecular mass was determined. The properties of pure polysaccharides were studied by ultraviolet, infrared, optical rotation, complete acid hydrolysis, periodic acid oxidation and Smith degradation, partial acid hydrolysis and methylation analysis . Results: A polysaccharide pure component LTC-Ⅱ was obtained with a relative molecular mass of 22 000 Da. The monosaccharide consisted of arabinose, mannose, glucose and galactose in a molar ratio of 35.2: 1.0: 13.4: 4.2. The LTC-II backbone consisted of mannose, glucose and galactose linked Glucose-based branching side chains consist of arabinose, glucose and galactose, with (1 → 5) linked arabinose, glucose and galactose at the molecular end. CONCLUSION: The chemical structure of LTC-Ⅱ of the wintergall polysaccharide was first studied in depth.