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目的探讨黄芩苷、黄芩素对变形链球菌生物膜形成的影响,为中药生物膜抑制剂的研究开发提供一定的基础。方法以变形链球菌10556为研究对象,以氯已定为阳性对照药,测试黄芩苷、黄芩素对变形链球菌的最低抑制浓度(MIC);采用倍比稀释法对生物膜内活菌菌落计数;以原子力显微镜和扫描电镜观察其对生物膜形态结构的影响;结晶紫染色法测定对生物膜的抑制率。结果形态学观察可见生物膜被破坏,基质样物变稀疏,细菌群聚大为降低;同时,黄芩苷、黄芩素对变形链球菌生物膜形成具有明显的抑制作用。结论黄芩苷、黄芩素具有明显的体外抑菌作用,该结果可为中药黄芩抗菌作用的物质基础研究提供依据。
Objective To investigate the effects of baicalin and baicalein on the biofilm formation of Streptococcus mutans and provide a basis for the research and development of biofilm inhibitors. Methods Streptococcus mutans 10556 was used as the research object. Chlorhexidine was used as a positive control drug to test the minimum inhibitory concentration (MIC) of baicalin and baicalein against Streptococcus mutans. The viable bacteria colonies were counted by bi-specific dilution Atomic force microscopy and scanning electron microscopy were used to observe the effect of biofilm morphological structure. The inhibition rate of biofilm was determined by crystal violet staining. Results Morphological observation revealed that the biofilm was destroyed, the matrix samples became sparse and the bacterial population was greatly reduced. Meanwhile, baicalin and baicalein significantly inhibited the biofilm formation of Streptococcus mutans. Conclusion Baicalin and baicalein have obvious antibacterial activity in vitro. This result may provide the basis for the material basis of the antibacterial effect of Scutellaria baicalensis Georgi.