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采用蛭石栽培,在100mmol·L-1NaCl胁迫下,对耐盐性不同的两个品种菜用大豆种子的丙二醛(MDA)含量和多胺(PAs)代谢进行了研究.结果表明:NaCl胁迫显著增加了菜用大豆种子的MDA含量,但耐盐品种‘绿领特早’(LL)的增幅低于盐敏感品种‘理想高产95-1’(LX).与LX相比,LL种子在整个NaCl胁迫期间均维持了相对较高的游离态精胺(Spm)、结合态Spm、结合态亚精胺(Spd)、束缚态Spd和束缚态腐胺(Put)含量,较高的(Spd+Spm)/Put和(cPAs+bPAs)/fPAs值及较低的Put/PAs值,在胁迫中、后期(9～15d)维持了相对较高的游离态Spd含量;胁迫期间,LL的精胺酸脱羧酶(ADC)长时期(6～15d)保持相对较高的活性,而多胺氧化酶(PAO)则长时期(6～15d)维持相对较低的活性.综上,LL具有较强的多胺合成能力及较强的Put向Spd和Spm以及游离态多胺向结合态和束缚态多胺转化的能力,进而有效抑制了细胞的膜脂过氧化,这可能是其耐盐性较强的重要原因之一.
Under the stress of 100mmol·L-1NaCl, malondialdehyde (MDA) content and polyamines (PAs) metabolism of two soybean cultivars with different salt tolerance were studied by using vermiculite cultivation.The results showed that NaCl Stress increased the content of MDA in vegetable soybean significantly, but the growth rate of salt-tolerant variety “LL” was lower than that of salt-sensitive variety ’L 95.’ Compared with LX, LL seed Spd, bound Spd, bound Spd and bound putrescine (Put), higher (Spd + Spm) / Put and (cPAs + bPAs) / fPAs values and lower Put / PAs values. During the stress period, the relatively high content of free Spd was maintained during the late stage (9-15 days) Acid decarboxylase (ADC) maintained relatively high activity for a long time (6 ~ 15 days), whereas polyamine oxidase (PAO) maintained relatively low activity for a long time (6 ~ 15 days) Polyamines synthesis ability and strong Put to Spd and Spm and free polyamines to bound and bound polyamines conversion ability, thereby effectively inhibiting the membrane lipid peroxidation, which may be its resistance One of the important reasons strong.