为阐明同型半胱氨酸与神经管畸形之间的关系,本实验应用鸡胚培养、尼罗兰盐活体染色、扫描电镜和光镜观察方法探索了HCY对神经胚形成期胚胎(2天龄)的发育毒性和致崎作用。结果显示随着HCY浓度的增高(0～80mM),NTDs发生率由0增至到25.8％,呈剂量一反应关系;神经系统是HCY作用的主要靶器官,具体表现为露脑、脊柱裂、神经管未闭和脑形态异常;本文还从剂量反应关系、细胞死亡、羊膜受损等方面初步分析了HCY的可能致畸机理。 In order to elucidate the relationship between homocysteine ​​and neural tube defects, we used chick embryo culture, livelihood Nileran salt staining, scanning electron microscopy and light microscopy to explore the effects of HCY on neurogenic embryos (2 days old) Developmental Toxicity and Intoxication. The results showed that with the increase of HCY concentration (0 ~ 80mM), the incidence of NTDs increased from 0 to 25.8% in a dose-response relationship. The nervous system was the main target organ of HCY, Neural tube closure and abnormal brain morphology; this article also from dose-response relationship, cell death, amniotic damage and other aspects of possible teratogenic mechanism of HCY.