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目的观察硬膜外不同浓度辣椒辣素(CAP)对兔痛阈、神经功能和脊髓神经元结构的影响。方法健康新西兰雄性白兔36只,随机分为4组:对照组、C1、C2、C3组,每组9只。L6,7间隙硬膜外穿刺置管,C1、C2、C3组分别注入0．1％、0．25％、0．5％CAP 1 ml,对照组注入0．9生理盐水1 ml。观察后肢热痛阈和神经功能改变,注药后24 h,每组处死3只家兔,在光镜、电镜下观察L6,7脊髓神经元的病理改变。结果与对照组相比,注药后C1、C2、C3组痛阈延长(P<0．05),随CAP浓度增加,痛阈延长时间增加,而神经功能评分下降。光镜、电镜下显示C2组神经元发生可逆性改变,C3组发生不可逆性改变。结论兔硬膜外注入CAP可延长痛阈,高浓度CAP可导致神经功能和脊髓神经元结构损害。
Objective To observe the effects of epidural different concentrations of capsaicin (CAP) on the rabbit’s pain threshold, nerve function and spinal cord neuronal structure. Methods 36 New Zealand male white rabbits were randomly divided into 4 groups: control group, C1, C2, and C3 groups, with 9 mice in each group. In L6,7 space epidural puncture catheters, C1, C2, and C3 groups were injected with 0.1%, 0.25%, and 0.5% CAP 1 ml, respectively, and the control group was injected with 0.9 ml saline 0.9 ml. Observing the thermal threshold and neural function changes of the hindlimbs, 24 rabbits were killed in each group 24 hours after injection. The pathological changes of L6,7 spinal cord neurons were observed under light and electron microscope. Results Compared with the control group, the pain threshold of C1, C2, and C3 groups was prolonged after injection (P<0.05). With the increase of CAP concentration, the extension of pain threshold increased and the neurological function score decreased. Light microscopy and electron microscopy showed reversible changes in C2 neurons and irreversible changes in C3 group. Conclusion Epidural injection of CAP in rabbits can prolong the pain threshold, and high concentration of CAP can lead to neurological function and structural damage of spinal cord neurons.