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甘草的主要活性成分有甘草素(gly-cyrrhizin)或甘草酸(glycyrrhizic acid,GL),18α-甘草次酸(18α—Glucurrhetihic acid,18α-GA),和18β-甘草次酸(18β-Glycyrrhetimicacid,18β-GA)。本文报道采用一次分析步骤同时测定甘草提取物中GL,18α-GA和18β-GA含量的方法。仪器:HPLC系统由U_(6k)型进样器,2个510型泵,441型检测器,990型光电二极管阵列检测器和740型积分仪组成,洗脱在具有保护栓的Lichrospher 100RP-18(5μm)的封端栓(125×4mml.D.E.Merck)上进行。检测
The main active ingredients of licorice are glycyrrhizin (glycyrrhizin) or glycyrrhizic acid (GL), 18α-Glucurhetihic acid (18α-GA), and 18β-Glycyrrhetimic acid (18β-Glycyrrhetimic acid). 18β-GA). This article reports a method for the simultaneous determination of GL, 18α-GA and 18β-GA in licorice extract using a single analytical procedure. Instrument: The HPLC system consists of a U_(6k) injector, two 510 pumps, a 441 detector, a 990 photodiode array detector, and a 740 integrator, eluting on a Lichrospher 100RP-18 with a protective plug. This was performed on a (5 μm) capped plug (125×4 mml.DE Merck). Testing