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目的 探讨p2 1、CD44v蛋白在肺癌中可能的临床意义及相互间的关系。方法 采用流式细胞术 (FCM )检测 2 9例冻存的肺癌及 10例相应远离肿瘤部位的正常肺组织中p2 1、CD44v蛋白的表达。结果 肺癌组织中p2 1、CD44v的阳性率分别为 75 .9%和 5 1.7% ,两者相对含量 (FI值 )和阳性率均明显高于远癌肺组织 (P均 <0 .0 1)。p2 1和CD44v蛋白的表达明显相关 (P <0 .0 1)。p2 1的表达与肺癌分型、原发肿瘤大小、淋巴结转移、分期、分化程度等临床指标之间均无明显关系。CD44v表达在肺癌Ⅱ +Ⅲ期 ( 83 .3 % )高于Ⅰ期 ( 2 9.4% ) (P <0 .0 1) ,在伴有淋巴结转移的原发性肺癌 ( 81.8% )高于不伴有转移的肺癌 ( 3 3 .3 % ) (P <0 .0 5 )。结论 p2 1、CD44v异常表达在肺癌中可能具有一定的协同作用。CD44v阳性可能预示肺癌发生淋巴结转移的倾向 ,具有一定的临床意义。
Objective To investigate the possible clinical significance of p21 and CD44v proteins in lung cancer and their relationship with each other. Methods Flow cytometry (FCM) was used to detect the expression of p21 and CD44v proteins in 29 frozen lung cancer tissues and 10 normal lung tissues away from the tumor site. Results The positive rates of p21 and CD44v in lung cancer tissues were 75.9% and 51.7%, respectively. The relative content (FI value) and the positive rate of them in lung cancer tissues were significantly higher than those in distant lung cancer tissues (all P <0.01) . The expression of p2 1 and CD44v protein were significantly correlated (P <0.01). There was no significant correlation between the expression of p21 and clinical indicators such as lung cancer type, primary tumor size, lymph node metastasis, staging and differentiation degree. The expression of CD44v was significantly higher in patients with stage Ⅱ + Ⅲ lung cancer (83.3%) than in stage Ⅰ (2 9.4%) (P0.01), but higher in primary lung cancer with lymph node metastasis (81.8%) than without Metastatic lung cancer (33.3%) (P <0.05). Conclusion Abnormal expressions of p2 1 and CD44v may have some synergistic effects in lung cancer. The positive of CD44v may indicate the tendency of lymph node metastasis in lung cancer, which has some clinical significance.