割手密(Saccharum spontaneum L.)是甘蔗栽培品种的原始亲本之一,当前仍是甘蔗遗传改良的重要种质资源,为了对割手密的遗传多样性进行鉴定,本研究运用ISSR分子标记的方法,测定了24份割手密的遗传多样性,结果表明:14个ISSR引物共扩增出227条带,其中多态性条带为224条,多态性百分率为98.67%;24份材料的Nei指数(h)介于0.27~0.94,遗传相似系数集中在0.3~0.5之间;基于UPGMA聚类分析,在遗传距离0.70处,可以将24个材料分成4个类群;说明割手密有着相对丰富的遗传多样性,能够广泛用于甘蔗的遗传育种改良。 Saccharum spontaneum L., one of the original parents of sugarcane cultivars, is still an important germplasm resource for genetic improvement of sugarcane. In order to identify the genetic diversity of Saccharomyces cerevisiae, ISSR molecular marker The results showed that 227 bands were amplified by 14 ISSR primers, of which 224 were polymorphic and the percentage of polymorphism was 98.67%. Twenty-four materials The Nei index (h) ranged from 0.27 to 0.94 and the genetic similarity coefficient ranged from 0.3 to 0.5. Based on UPGMA cluster analysis, 24 materials could be divided into four groups at the genetic distance of 0.70. Relatively rich genetic diversity, can be widely used for genetic improvement of sugarcane.