自2002年后半年开始,汕尾出现大规模九孔鲍鲍苗脱板问题。为了解养殖环境中的细菌(主要是弧菌)对鲍苗的影响,于汕尾一养殖场跟踪观察多次鲍鱼育苗过程,取鲍苗正处于脱板过程中的水样和采苗板样用TCBS平板分离出25株菌,测定其分泌酪蛋白酶、胰蛋白酶和明胶酶3种胞外酶的能力,并进行其溶血现象分析。结果表明,产酶菌株达到17株,其中10株菌兼具分泌溶血素的能力。对这10株菌进行了种类鉴定,表明它们是副溶血弧菌(4株)、溶藻弧菌(2株)、腐败希瓦菌(3株)和杀鲑气单胞菌(1株)。 Since the second half of 2002, Shanwei has emerged as a large-scale nine-hole abalone vaccine. In order to understand the impact of bacteria (mainly Vibrio) in the breeding environment on the abalone seedlings, several abalone seedling growing processes were observed and observed in a farms in Shanwei. The abalone seedlings were taken water samples Twenty-five isolates were isolated from TCBS plates and their ability of secreting three extracellular enzymes, such as casein, trypsin and gelatinase, was determined and the hemolysis was analyzed. The results showed that there were 17 strains producing enzymes, of which 10 strains were both capable of secreting hemolysin. The 10 strains were identified as V. parahaemolyticus (4 strains), Vibrio alginolyticus (2 strains), Shewanella (3 strains) and Aeromonas salmonicola (1 strain) .