论文部分内容阅读
目的：观察肿瘤坏死因子（ＴＮＦ）单抗及一氧化氮（ＮＯ）抑制剂对脾切除小鼠内毒素攻击的治疗效应。方法：采用小鼠脾切除后内毒素攻击模型。结果：相同剂量内毒素（５ｍｇ／ｋｇ）攻击后，无脾动物死亡率明显高于有脾动物（Ｐ＜０．０１）；注射内毒素后５ｍｉｎ给予ＴＮＦ单抗治疗组，无脾动物死亡率明显降低（Ｐ＜０．０１）。应用ＮＯ抑制剂Ｌ－ＮＭＭＡ２ｍｇ／ｋｇ时，无脾动物死亡率明显降低（Ｐ＜０．０５）；但采用５ｍｇ／ｋｇ治疗时，死亡率则明显增高。结论：脾切除后动物凶险性感染的本质可能与ＴＮＦ大量生成及过量释放有关。ＴＮＦ拮抗剂及适当剂量的ＮＯ抑制剂对无脾动物凶险性感染的预防及治疗是有益的，而过量的ＮＯ抑制剂是不利的
Objective: To observe the therapeutic effect of tumor necrosis factor (TNF) monoclonal antibody and nitric oxide (NO) inhibitor on endotoxin attack in mice with splenectomy. Methods: The mouse model of endotoxin challenge after splenectomy was used. Results: After the same dose of endotoxin (5 mg / kg) challenge, the rate of death in spleenless animals was significantly higher than that in spleen animals (P <0.01) Significantly lower (P <0.01). When NO-inhibitor L-NMMA2mg / kg was used, the death rate of spleen-free animals was significantly decreased (P <0.05); however, the mortality rate was significantly increased when treated with 5mg / kg L-NMMA. Conclusion: The nature of dangerous animal infection after splenectomy may be related to the massive production of TNF and excessive release. TNF antagonists and appropriate doses of NO inhibitors are beneficial for the prevention and treatment of dangerous infections in spleenless animals, whereas excessive NO inhibitors are detrimental