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目的：研究血清循环miR-21在胃癌患者外周血中的表达以及临床意义。方法用实时定量逆转录聚合酶链式反应(qRT-PCR)分别检测60例胃癌患者、15例正常对照者外周血中miR-21的表达水平，了解miR-21表达与临床病理指标的关系。同时测定60例胃癌患者术后3d的miR-21的表达水平，了解其变化情况。结果胃癌患者术前血清中的miR-21表达水平明显高于正常对照者(﹤0.01)。 miR-21表达与患者性别、年龄及肿瘤部位差异均无显著性(均>0.05)，与浸润深度、TNM分期、淋巴结转移相关(均﹤0.05).术后水平较术前明显下降(﹤0.05)。结论血清miR-21在胃癌患者中表达上调，可能对胃癌的诊断具有一定的临床价值。“,”Objective To study the expression and clinical significance of circulating of miR-21in peripheral blood in patients with gastric cancer. Methods Peripheral blood samples were col ected from 60 preoperative patients with gastric cancer and 15 healthy volunteers. Steploop real-time reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the level of miR-21 . 60 serum samples were col ected from these patients 3 days after operation to examine the changes of miR-21 expression. Results The level of serum miR-21 in gastric cancer patients was significantly higher than those in health controls( ﹤0.01).The miR-21 level was associated with the lymph node metastasis, TNM stage( ﹤0.05, respectively), was not associated with age, sex or location of lesions ( > 0.05, respectively). The level of serum miR-21 significantly decreased after the operation compared with its preoperative level ( ﹤0.01).Conclusion Serum miR-21 is up-regulated in gastric cancer patients, suggesting its potential value for the diagnosis of gastric cancer.