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目的:研究Hepsin基因对前列腺癌PC3细胞生物学特性的影响。方法:用脂质体介导的基因转染技术,将pHepsin-IRES2质粒转染前列腺癌PC3细胞,用G418(600mg/L)筛选出阳性克隆。实时PCR检测转染前后Hepsin表达;MTT法和BrdU法检测细胞生长曲线;Boyden小室和划痕法观察转染前后细胞侵袭力的变化。结果:建立稳定表达Hepsin基因的PC3细胞系;转染Hepsin基因的PC3细胞的生长速度和转染空载体的对照组没有显著差异;转染Hepsin基因后前列腺癌PC3细胞的侵袭力显著增加(P<0.05)。结论:Hepsin基因对前列腺癌PC3细胞的增殖无显著作用,可明显促进PC3细胞的侵袭力。
Objective: To study the effect of Hepsin gene on biological characteristics of prostate cancer PC3 cells. Methods: Lipofectamine-mediated gene transfection was used to transfect pHepsin-IRES2 plasmid into prostate cancer PC3 cells. The positive clones were screened by G418 (600 mg / L). Real-time PCR was used to detect the expression of Hepsin before and after transfection. MTT assay and BrdU assay were used to detect the cell growth curve. Boyden chamber and scratch method were used to observe the changes of invasiveness of cells before and after transfection. RESULTS: The PC3 cell line stably expressing Hepsin gene was established. The growth rate of PC3 cells transfected with Hepsin gene was not significantly different from that of control cells transfected with empty vector. The invasiveness of PC3 cells transfected with Hepsin gene was significantly increased (P <0.05). Conclusion: The Hepsin gene has no significant effect on the proliferation of prostate cancer PC3 cells, and can significantly promote the invasiveness of PC3 cells.