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目的 研究乐果急性染毒对大鼠脑M1、M2 受体的影响。方法 2 4只SD大鼠随机分为 4组 ,采用皮下注射染毒 ,乐果剂量分别为 0、2 5、5 0、10 0mg/kg。染毒后 4 8h取大鼠脑组织 ,采用放射结合法分析M1、M2 受体数目和亲和力。结果 乐果各剂量染毒组大鼠胆碱能M1、M2 亚型的受体密度均低于对照组 ,其中 10 0mg/kg组大鼠脑中M1受体密度为 (2 4 4 .4± 81.4 )fmol/mgpro ,同对照组 [(44 0 .0± 95 .0 )fmol/mgpro]相比 ,差异有显著性 (P <0 .0 5 ) ,M2 受体密度与对照组的差异无显著性 (P >0 .0 5 )。染毒组大鼠脑M1、M2 受体亲和力均高于对照组 ,但差异无显著性 (P >0 .0 5 )。相关分析发现 ,脑组织中胆碱酯酶 (ChE)活力与M1受体密度相关 (r=0 .5 83,P <0 .0 1)。结论 急性乐果染毒后大鼠脑中M1、M2 受体密度有下降趋势 ,可能与ChE活力改变有关。提示机体胆碱能亢进症状减轻可能与大鼠脑中的M1、M2 受体数目的减少有关
Objective To study the effects of acute dimethoate exposure on M1 and M2 receptors in rat brain. Methods Twenty-four SD rats were randomly divided into 4 groups. The mice were injected subcutaneously with the doses of 0, 25, 50 and 100 mg / kg, respectively. 48 h after exposure to rat brain tissue taken by radioimmunoassay analysis M1, M2 receptor number and affinity. Results The receptor density of M1 and M2 subtypes of rats in each dose group of dimethoate was lower than that of the control group, and M1 receptor density in the rat brain of 10 mg / kg group was (24.4 ± 0.4) 81.4) fmol / mgpro, compared with the control group [(44.0 ± 95.0) fmol / mgpro], the difference was significant (P <0.05), the difference of M2 receptor density with the control group Significance (P> 0.05). The affinity of M1 and M2 receptors in the brain of rats in the exposure group were higher than those in the control group, but the difference was not significant (P> 0.05). Correlation analysis showed that the activity of ChE in brain tissue correlated with M1 receptor density (r = 0.583, P <0.01). Conclusion The concentrations of M1 and M2 receptors in rat brain after acute dimethoate exposure show a decreasing trend, which may be related to the change of ChE activity. Suggesting that the alleviation of cholinergic symptoms may be related to the reduction of the number of M1 and M2 receptors in rat brain