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应用聚丙烯酰胺凝胶电泳(PAGE)法对取自福建宁德官井洋海区养殖群体和野生种群各20尾大黄鱼的9种同工酶15个基因座位进行检测分析。结果表明,大黄鱼眼球中乳酸脱氢酶(LDH)酶谱表现为LDH的经典模式即有5种谱型的LDH。在所检测出的15个基因座位中 ,野生种群样品的编码苹果酸脱氢酶(sMDH)、苹果酸酶(ME)和琥珀酸脱氢酶(IDDH 1)的3个基因座位具有多态性 ;养殖群体中仅发现1个基因座位具有多态性(IDDH 1)。所检测的野生种群比养殖群体的遗传变异水平高。但从总体上来说 ,官井洋大黄鱼遗传多样性相对较低 ,表明该种群已出现了种质退化现象。因此 ,进行科学的遗传管理对保护和恢复大黄鱼资源是相当必要的。
Polyacrylamide gel electrophoresis (PAGE) was used to detect the 15 loci of 9 isozymes in cultured and wild populations of 20 large yellow croaker collected from Gujingyanghai of Ningde, Fujian Province. The results showed that the lactate dehydrogenase (LDH) enzyme spectrum of the large yellow croaker showed LDH classic pattern that there are five types of LDH. Among the 15 loci tested, wild-type samples had polymorphisms in the three loci encoding malate dehydrogenase (sMDH), malate (ME) and succinate dehydrogenase (IDDH 1) ; Only one locus was found polymorphically (IDDH 1) in the cultured population. The detected wild populations have a higher level of genetic variation than the cultured populations. However, on the whole, the genetic diversity of the large yellow croaker of Gujingyang is relatively low, indicating that germplasm degeneration has occurred in this population. Therefore, scientific genetic management is necessary to protect and restore the resources of big yellow croaker.