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本文全面总结了雷州林业局林科所桉树组培技术,提出了外植体3次高效消毒的新方法;同时阐明了茅诱导时茎段采集的最佳季节和最佳部位。为了提高芽增殖系数和诱导生根率,对继代培养基和生根培养基进行筛选,尤其是对继代培养基筛选研究己达到按不同树种和无性系选用最佳培养基的程度。
In this paper, we summarized the tissue culture technology of Eucalyptus in Linkee Institute of Leizhou Forestry Bureau, and put forward a new method of high efficiency disinfection of explants three times. At the same time, the best seasons and the best parts of stems collection were elucidated. In order to improve the bud multiplication coefficient and the induction of rooting rate, the subculture medium and rooting medium were screened, especially for the screening of subculture medium to the extent that the best medium for different tree species and clones was selected.